Content and location of lymphocyte subpopulations with markers CD4+, CD8+ and CD20+ in the esophageal tonsil of chickens and the Meckel diverticulum of ducks

Immune formations of birds' digestive organs, including the esophageal tonsil and Meckel’s diverticulum, protect body from foreign antigens that enter with food water play an important role in maintaining genetic constancy its internal environment. This unique property immune system is formed during ontogenesis associated selection lymphocyte clones are able to respond carry out a specific response two types: humoral cellular. article presents results study T- B-lymphocyte subpopulations Shever 579 cross chickens at age 25, 180 300 days, Meckel diverticulum Blagovarsky ducks 30, 150 days. Immunohistochemical statistical research methods were used determine localization quantitative parameters cell populations lymphocytes (CD4+, CD8+, CD20+) using monoclonal antibodies DAKO EnVision FLEX+ imaging (Dako Cytomation, Denmark). Separate T-lymphocytes (CD4+ - helpers, CD8+ -cytotoxic / T-suppressors) mature B-lymphocytes (CD20+) found birds. Their presence confirms antigen-independent proliferation differentiation into effector cells occur system. The lymphoid tissue these represented mainly by well-defined diffuse form nodules light centers (secondary). In chickens, structures located tunica mucosa tela submucosa, – also muscularis. content markers predominates compared secondary nodules. tonsil, near adenomeres excretory ducts glands, blood vessels, under surface epithelium, around crypts, their epithelium villi. They on periphery. indices indicated which we determined characteristics poultry postnatal period ontogenesis. According our observations, CD20+ was highest, while CD4+ much smaller. indicates increase activity predominance immunity over cellular one. highest birds is, sexual maturity. data presented work can be morphologists researching organs system, immunologists, specialists involved breeding, raising educational work.